Translational_Unit

Part:BBa_K4879009:Design

Designed by: Anuvind Pramod   Group: iGEM23_IISER-Pune-India   (2023-10-08)


Yarrowia lipolytica HygR construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1561
    Illegal suffix found in sequence at 1831
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1825
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1561
    Illegal EcoRI site found at 1825
    Illegal SpeI site found at 1832
    Illegal PstI site found at 1846
    Illegal NotI site found at 1567
    Illegal NotI site found at 1839
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1561
    Illegal EcoRI site found at 1825
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1561
    Illegal suffix found in sequence at 1832
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1825
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1561
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1825
    Illegal XbaI site found at 1576
    Illegal SpeI site found at 1832
    Illegal PstI site found at 1846
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression cassette contained upstream and downstream sequences homologous to the faa1 gene locus, in order to delete the gene by homologous recombination. This works by replacing the faa1 gene present in between the homology region in the genome with the hygromycin construct.


Source

The expression construct was synthesised by IDT.


References